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sheep polyclonal foxc2  (R&D Systems Hematology)

 
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    Structured Review

    R&D Systems Hematology sheep polyclonal foxc2
    (A) Overlay of Mesp1 , <t>Foxc2</t> , and Ai14 Mesp1 -lineage gene expression in cell types of Middle mesoderm atlas UMAP. (B) Immunostaining and Light Sheet Confocal microscopy for Foxc2 (magenta), Smarcd3 -F6 (green) and Mesp1 via Cre detection (blue) in Middle stage embryos (∼E6.75). Arrowheads denote domain boundaries in control and disruption in Mesp1 KO embryo. Scale bars are 100 μm.
    Sheep Polyclonal Foxc2, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheep+polyclonal+foxc2/Mouse+FoxC2+Antibody/bio_rxiv__2022__08__22__504863-383-5-8
    Average 93 stars, based on 46 article reviews
    sheep polyclonal foxc2 - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors"

    Article Title: A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors

    Journal: bioRxiv

    doi: 10.1101/2022.08.22.504863

    (A) Overlay of Mesp1 , Foxc2 , and Ai14 Mesp1 -lineage gene expression in cell types of Middle mesoderm atlas UMAP. (B) Immunostaining and Light Sheet Confocal microscopy for Foxc2 (magenta), Smarcd3 -F6 (green) and Mesp1 via Cre detection (blue) in Middle stage embryos (∼E6.75). Arrowheads denote domain boundaries in control and disruption in Mesp1 KO embryo. Scale bars are 100 μm.
    Figure Legend Snippet: (A) Overlay of Mesp1 , Foxc2 , and Ai14 Mesp1 -lineage gene expression in cell types of Middle mesoderm atlas UMAP. (B) Immunostaining and Light Sheet Confocal microscopy for Foxc2 (magenta), Smarcd3 -F6 (green) and Mesp1 via Cre detection (blue) in Middle stage embryos (∼E6.75). Arrowheads denote domain boundaries in control and disruption in Mesp1 KO embryo. Scale bars are 100 μm.

    Techniques Used: Gene Expression, Immunostaining, Confocal Microscopy, Control, Disruption

    Related Articles

    Gene Expression:

    Article Title: A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors
    Article Snippet: All steps of immunostaining protocol were done at 37°C with gentle rocking and rotation.All steps of immunostaining protocol were done at 37°C with gentle rocking and rotation.. Antibodies used in this study: sheep polyclonal Foxc2 (R&D, AF6989), chicken polyclonal GFP (Aves, GFP-1020), rabbit polyclonal Cre (Millipore, 69050).. Light sheet embryo images were acquired using Z1 Light Sheet Microscope (Zeiss) and processed as described ( ).Light sheet embryo images were acquired using Z1 Light Sheet Microscope (Zeiss) and processed as described ( ).

    Immunostaining:

    Article Title: A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors
    Article Snippet: All steps of immunostaining protocol were done at 37°C with gentle rocking and rotation.All steps of immunostaining protocol were done at 37°C with gentle rocking and rotation.. Antibodies used in this study: sheep polyclonal Foxc2 (R&D, AF6989), chicken polyclonal GFP (Aves, GFP-1020), rabbit polyclonal Cre (Millipore, 69050).. Light sheet embryo images were acquired using Z1 Light Sheet Microscope (Zeiss) and processed as described ( ).Light sheet embryo images were acquired using Z1 Light Sheet Microscope (Zeiss) and processed as described ( ).

    Confocal Microscopy:

    Article Title: A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors
    Article Snippet: All steps of immunostaining protocol were done at 37°C with gentle rocking and rotation.All steps of immunostaining protocol were done at 37°C with gentle rocking and rotation.. Antibodies used in this study: sheep polyclonal Foxc2 (R&D, AF6989), chicken polyclonal GFP (Aves, GFP-1020), rabbit polyclonal Cre (Millipore, 69050).. Light sheet embryo images were acquired using Z1 Light Sheet Microscope (Zeiss) and processed as described ( ).Light sheet embryo images were acquired using Z1 Light Sheet Microscope (Zeiss) and processed as described ( ).

    Control:

    Article Title: A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors
    Article Snippet: All steps of immunostaining protocol were done at 37°C with gentle rocking and rotation.All steps of immunostaining protocol were done at 37°C with gentle rocking and rotation.. Antibodies used in this study: sheep polyclonal Foxc2 (R&D, AF6989), chicken polyclonal GFP (Aves, GFP-1020), rabbit polyclonal Cre (Millipore, 69050).. Light sheet embryo images were acquired using Z1 Light Sheet Microscope (Zeiss) and processed as described ( ).Light sheet embryo images were acquired using Z1 Light Sheet Microscope (Zeiss) and processed as described ( ).

    Disruption:

    Article Title: A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors
    Article Snippet: All steps of immunostaining protocol were done at 37°C with gentle rocking and rotation.All steps of immunostaining protocol were done at 37°C with gentle rocking and rotation.. Antibodies used in this study: sheep polyclonal Foxc2 (R&D, AF6989), chicken polyclonal GFP (Aves, GFP-1020), rabbit polyclonal Cre (Millipore, 69050).. Light sheet embryo images were acquired using Z1 Light Sheet Microscope (Zeiss) and processed as described ( ).Light sheet embryo images were acquired using Z1 Light Sheet Microscope (Zeiss) and processed as described ( ).



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    Transcriptional profiles of cardiac mesoderm in Mesp1 KO embryos. (A,F,K) scRNA-seq UMAP atlases of Ai14, Smarcd3 -F6 double-positive mesoderm cells from early (A; 5504 cells), middle (F; 7666 cells) and late (K; 22,622 cells) developmental embryo stages. (B,C,G,H,L,M) Associated UMAPs colored by genotype (B,G,L) and Smarcd3 -F6-eGFP expression (C,H,M). (D,E) Early mesoderm DGE in LSMeso2 (D) and EomesPSMeso (E). (I,J) Middle mesoderm DGE in LSMeso2 (I) and Mesp1ME (J). (N-P) Late mesoderm DGE in Meso2 (N), Meso1 (O), and postLPM (P). Significant changes denoted with adjusted P <0.05. antPrSoM, anterior presomitic mesoderm; DE-ME, definitive endoderm/mesendoderm; Foxc2M, <t>Foxc2</t> + mesoderm; Meso, mesoderm; PSMeso, primitive streak mesoderm; PS-NM, primitive streak/neuromesodermal.
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    Image Search Results


    Transcriptional profiles of cardiac mesoderm in Mesp1 KO embryos. (A,F,K) scRNA-seq UMAP atlases of Ai14, Smarcd3 -F6 double-positive mesoderm cells from early (A; 5504 cells), middle (F; 7666 cells) and late (K; 22,622 cells) developmental embryo stages. (B,C,G,H,L,M) Associated UMAPs colored by genotype (B,G,L) and Smarcd3 -F6-eGFP expression (C,H,M). (D,E) Early mesoderm DGE in LSMeso2 (D) and EomesPSMeso (E). (I,J) Middle mesoderm DGE in LSMeso2 (I) and Mesp1ME (J). (N-P) Late mesoderm DGE in Meso2 (N), Meso1 (O), and postLPM (P). Significant changes denoted with adjusted P <0.05. antPrSoM, anterior presomitic mesoderm; DE-ME, definitive endoderm/mesendoderm; Foxc2M, Foxc2 + mesoderm; Meso, mesoderm; PSMeso, primitive streak mesoderm; PS-NM, primitive streak/neuromesodermal.

    Journal: Development (Cambridge, England)

    Article Title: A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors

    doi: 10.1242/dev.201229

    Figure Lengend Snippet: Transcriptional profiles of cardiac mesoderm in Mesp1 KO embryos. (A,F,K) scRNA-seq UMAP atlases of Ai14, Smarcd3 -F6 double-positive mesoderm cells from early (A; 5504 cells), middle (F; 7666 cells) and late (K; 22,622 cells) developmental embryo stages. (B,C,G,H,L,M) Associated UMAPs colored by genotype (B,G,L) and Smarcd3 -F6-eGFP expression (C,H,M). (D,E) Early mesoderm DGE in LSMeso2 (D) and EomesPSMeso (E). (I,J) Middle mesoderm DGE in LSMeso2 (I) and Mesp1ME (J). (N-P) Late mesoderm DGE in Meso2 (N), Meso1 (O), and postLPM (P). Significant changes denoted with adjusted P <0.05. antPrSoM, anterior presomitic mesoderm; DE-ME, definitive endoderm/mesendoderm; Foxc2M, Foxc2 + mesoderm; Meso, mesoderm; PSMeso, primitive streak mesoderm; PS-NM, primitive streak/neuromesodermal.

    Article Snippet: Antibodies used in this study were: sheep polyclonal Foxc2 (R&D Systems, AF6989), chicken polyclonal GFP (Aves Labs, GFP-1020), rabbit polyclonal Cre (Millipore, 69050).

    Techniques: Expressing

    (A) Overlay of Mesp1 , Foxc2 , and Ai14 Mesp1 -lineage gene expression in cell types of Middle mesoderm atlas UMAP. (B) Immunostaining and Light Sheet Confocal microscopy for Foxc2 (magenta), Smarcd3 -F6 (green) and Mesp1 via Cre detection (blue) in Middle stage embryos (∼E6.75). Arrowheads denote domain boundaries in control and disruption in Mesp1 KO embryo. Scale bars are 100 μm.

    Journal: bioRxiv

    Article Title: A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors

    doi: 10.1101/2022.08.22.504863

    Figure Lengend Snippet: (A) Overlay of Mesp1 , Foxc2 , and Ai14 Mesp1 -lineage gene expression in cell types of Middle mesoderm atlas UMAP. (B) Immunostaining and Light Sheet Confocal microscopy for Foxc2 (magenta), Smarcd3 -F6 (green) and Mesp1 via Cre detection (blue) in Middle stage embryos (∼E6.75). Arrowheads denote domain boundaries in control and disruption in Mesp1 KO embryo. Scale bars are 100 μm.

    Article Snippet: Antibodies used in this study: sheep polyclonal Foxc2 (R&D, AF6989), chicken polyclonal GFP (Aves, GFP-1020), rabbit polyclonal Cre (Millipore, 69050).

    Techniques: Gene Expression, Immunostaining, Confocal Microscopy, Control, Disruption